{"id":100,"date":"2023-02-08T02:55:09","date_gmt":"2023-02-08T02:55:09","guid":{"rendered":"http:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/"},"modified":"2023-03-30T00:11:59","modified_gmt":"2023-03-30T05:11:59","slug":"ultimate-3000-binary-analytical-liquid-chromatography-system","status":"publish","type":"page","link":"http:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/","title":{"rendered":"UltiMate 3000 Binary Analytical Liquid Chromatography System"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"100\" class=\"elementor elementor-100\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-06cbc4b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"06cbc4b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-32f0d63\" data-id=\"32f0d63\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9693bf1 elementor-widget elementor-widget-text-editor\" data-id=\"9693bf1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><img fetchpriority=\"high\" decoding=\"async\" class=\"size-full wp-image-757 image_responsive alignleft\" src=\"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg\" alt=\"UltiMate 3000 Binary Analytical Liquid Chromatography System\" width=\"300\" height=\"606\" srcset=\"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg 300w, http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system-149x300.jpg 149w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>The UltiMate 3000 UHPLC system (Thermo Scientific) is capable of precise and efficient offline fractionation of complex protein digests. This system is used in the facility workflow to fractionate peptides at basic pH in order to complement the acidic pH separation performed during liquid chromatography-mass spectrometry\/mass spectrometry (LC-MS\/MS) analysis.<\/p><p>The UltiMate 3000 can sustain pressures as high as 9000 psi and flow rates as low as 50 nL\/min. The system includes an analytical autosampler and fraction collector to allow efficient processing of large numbers of samples, as well as a UV detector to monitor peptide elution and prevent cross-contamination.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>The UltiMate 3000 UHPLC system (Thermo Scientific) is capable of precise and efficient offline fractionation of complex protein digests. This system is used in the facility workflow to fractionate peptides at basic pH in order to complement the acidic pH separation performed during liquid chromatography-mass spectrometry\/mass spectrometry (LC-MS\/MS) analysis. The UltiMate 3000 can sustain pressures [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":90,"menu_order":160,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.2 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>UltiMate 3000 Binary Analytical Liquid Chromatography System - IDEA National Resource for Quantitative Proteomics<\/title>\n<meta name=\"description\" content=\"The UltiMate 3000 Binary Analytical Liquid Chromatography System performs precise\/efficient offline fractionation of complex protein digests.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"UltiMate 3000 Binary Analytical Liquid Chromatography System\" \/>\n<meta property=\"og:description\" content=\"The UltiMate 3000 Binary Analytical Liquid Chromatography System performs precise\/efficient offline fractionation of complex protein digests.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/\" \/>\n<meta property=\"og:site_name\" content=\"IDEA National Resource for Quantitative Proteomics\" \/>\n<meta property=\"article:modified_time\" content=\"2023-03-30T05:11:59+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:title\" content=\"UltiMate 3000 Binary Analytical Liquid Chromatography System\" \/>\n<meta name=\"twitter:description\" content=\"The UltiMate 3000 Binary Analytical Liquid Chromatography System performs precise\/efficient offline fractionation of complex protein digests.\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/\",\"url\":\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/\",\"name\":\"UltiMate 3000 Binary Analytical Liquid Chromatography System - IDEA National Resource for Quantitative Proteomics\",\"isPartOf\":{\"@id\":\"https:\/\/idearesourceproteomics.org\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#primaryimage\"},\"thumbnailUrl\":\"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg\",\"datePublished\":\"2023-02-08T02:55:09+00:00\",\"dateModified\":\"2023-03-30T05:11:59+00:00\",\"description\":\"The UltiMate 3000 Binary Analytical Liquid Chromatography System performs precise\/efficient offline fractionation of complex protein digests.\",\"breadcrumb\":{\"@id\":\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#primaryimage\",\"url\":\"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg\",\"contentUrl\":\"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg\",\"width\":300,\"height\":606,\"caption\":\"UltiMate 3000 Binary Analytical Liquid Chromatography System\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"http:\/\/idearesourceproteomics.org\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Instrumentation\",\"item\":\"https:\/\/idearesourceproteomics.org\/instrumentation\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"UltiMate 3000 Binary Analytical Liquid Chromatography System\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/idearesourceproteomics.org\/#website\",\"url\":\"https:\/\/idearesourceproteomics.org\/\",\"name\":\"IDEA National Resource for Quantitative Proteomics\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/idearesourceproteomics.org\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/idearesourceproteomics.org\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/idearesourceproteomics.org\/#organization\",\"name\":\"IDEA National Resource for Quantitative Proteomics\",\"url\":\"https:\/\/idearesourceproteomics.org\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/idearesourceproteomics.org\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/idearesourceproteomics.org\/wp-content\/uploads\/idea-logo-hdr.png\",\"contentUrl\":\"https:\/\/idearesourceproteomics.org\/wp-content\/uploads\/idea-logo-hdr.png\",\"width\":270,\"height\":86,\"caption\":\"IDEA National Resource for Quantitative Proteomics\"},\"image\":{\"@id\":\"https:\/\/idearesourceproteomics.org\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"UltiMate 3000 Binary Analytical Liquid Chromatography System - IDEA National Resource for Quantitative Proteomics","description":"The UltiMate 3000 Binary Analytical Liquid Chromatography System performs precise\/efficient offline fractionation of complex protein digests.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/","og_locale":"en_US","og_type":"article","og_title":"UltiMate 3000 Binary Analytical Liquid Chromatography System","og_description":"The UltiMate 3000 Binary Analytical Liquid Chromatography System performs precise\/efficient offline fractionation of complex protein digests.","og_url":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/","og_site_name":"IDEA National Resource for Quantitative Proteomics","article_modified_time":"2023-03-30T05:11:59+00:00","og_image":[{"url":"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg"}],"twitter_card":"summary_large_image","twitter_title":"UltiMate 3000 Binary Analytical Liquid Chromatography System","twitter_description":"The UltiMate 3000 Binary Analytical Liquid Chromatography System performs precise\/efficient offline fractionation of complex protein digests.","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/","url":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/","name":"UltiMate 3000 Binary Analytical Liquid Chromatography System - IDEA National Resource for Quantitative Proteomics","isPartOf":{"@id":"https:\/\/idearesourceproteomics.org\/#website"},"primaryImageOfPage":{"@id":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#primaryimage"},"image":{"@id":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#primaryimage"},"thumbnailUrl":"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg","datePublished":"2023-02-08T02:55:09+00:00","dateModified":"2023-03-30T05:11:59+00:00","description":"The UltiMate 3000 Binary Analytical Liquid Chromatography System performs precise\/efficient offline fractionation of complex protein digests.","breadcrumb":{"@id":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#primaryimage","url":"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg","contentUrl":"http:\/\/idearesourceproteomics.org\/wp-content\/uploads\/ultiMate-3000-binary-analytical-liquid-chromatography-system.jpg","width":300,"height":606,"caption":"UltiMate 3000 Binary Analytical Liquid Chromatography System"},{"@type":"BreadcrumbList","@id":"https:\/\/idearesourceproteomics.org\/instrumentation\/ultimate-3000-binary-analytical-liquid-chromatography-system\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"http:\/\/idearesourceproteomics.org\/"},{"@type":"ListItem","position":2,"name":"Instrumentation","item":"https:\/\/idearesourceproteomics.org\/instrumentation\/"},{"@type":"ListItem","position":3,"name":"UltiMate 3000 Binary Analytical Liquid Chromatography System"}]},{"@type":"WebSite","@id":"https:\/\/idearesourceproteomics.org\/#website","url":"https:\/\/idearesourceproteomics.org\/","name":"IDEA National Resource for Quantitative Proteomics","description":"","publisher":{"@id":"https:\/\/idearesourceproteomics.org\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/idearesourceproteomics.org\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/idearesourceproteomics.org\/#organization","name":"IDEA National Resource for Quantitative Proteomics","url":"https:\/\/idearesourceproteomics.org\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/idearesourceproteomics.org\/#\/schema\/logo\/image\/","url":"https:\/\/idearesourceproteomics.org\/wp-content\/uploads\/idea-logo-hdr.png","contentUrl":"https:\/\/idearesourceproteomics.org\/wp-content\/uploads\/idea-logo-hdr.png","width":270,"height":86,"caption":"IDEA National Resource for Quantitative Proteomics"},"image":{"@id":"https:\/\/idearesourceproteomics.org\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/pages\/100"}],"collection":[{"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/comments?post=100"}],"version-history":[{"count":12,"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/pages\/100\/revisions"}],"predecessor-version":[{"id":1916,"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/pages\/100\/revisions\/1916"}],"up":[{"embeddable":true,"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/pages\/90"}],"wp:attachment":[{"href":"http:\/\/idearesourceproteomics.org\/wp-json\/wp\/v2\/media?parent=100"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}