Protocols

The resources below may be helpful for planning proteomics experiments. Proper sample preparation is critical to the success of any proteomics project. We strongly recommend consultation with mass spectrometrists and bioinformaticists before starting any experiment. IDeA Facility staff are happy to provide consultation and answer questions to assist with experimental design and execution. Please contact us at proteomics@uams.edu

Filter-Assisted Sample Preparation
https://www.biochem.mpg.de/226356/FASP

SDS-PAGE
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels.html

Serum/Plasma Depletion
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-mass-spectrometry-analysis/sample-prep-mass-spectrometry/abundant-protein-depletion-mass-spectrometry.html

Tandem Mass Tag (TMT) Labeling
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-mass-spectrometry-analysis/protein-quantitation-mass-spectrometry/tandem-mass-tag-systems.html

Peptide Enrichment and Fractionation
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-mass-spectrometry-analysis/sample-prep-mass-spectrometry/peptide-enrichment-fractionation-mass-spectrometry.html

Orbitrap Mass Spectrometry
https://planetorbitrap.com/

Protocols coming soon.